Data/hora: 04/06/2024 03:44:20

Provedor de dados: | 58
País: | Brazil
Título: | Kinetic properties of Streptomyces canarius L- Glutaminase and its anticancer efficiency
Autores: | Reda,Fifi M.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | L-glutaminase; Anti-cancer; Cytotoxicity; MTT assay.
Resumo: | Abstract L-glutaminase was produced by Streptomyces canarius FR (KC460654) with an apparent molecular mass of 44 kDa. It has 17.9 purification fold with a final specific activity 132.2 U/mg proteins and 28% yield recovery. The purified L-glutaminase showed a maximal activity against L-glutamine when incubated at pH 8.0 at 40 °C for 30 min. It maintained its stability at wide range of pH from 5.0 11.0 and thermal stable up to 60 °C with Tm value 57.5 °C. It has high affinity and catalytic activity for L-glutamine (Km 0.129 mM, Vmax 2.02 U/mg/min), followed by L-asparagine and L-aspartic acid. In vivo, L-glutaminase showed no observed changes in liver; kidney functions; hematological parameters and slight effect on RBCs and level of platelets after 10 days of rabbit's injection. The anticancer activity of L-glutaminase was also tested against five types of human cancer cell lines using MTT assay in vitro. L-glutaminase has a significant efficiency against Hep-G2 cell (IC50, 6.8 μg/mL) and HeLa cells (IC50, 8.3 μg/mL), while the growth of MCF-7 cells was not affected. L-glutaminase has a moderate cytotoxic effect against HCT-116 cell (IC50, 64.7 μg/mL) and RAW 264.7 cell (IC50, 59.3 μg/mL).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400957
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-838246420130847
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.46 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess