Data/hora: 16/06/2024 09:27:22

Provedor de dados: 	Brazilian Journal of Microbiology	
País: 	Brazil	
Título: 	Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy	
Autores: 	Silva,Daiani M.; Batista,Luís R.; Rezende,Elisângela F.; Fungaro,Maria Helena P.; Sartori,Daniele; Alves,Eduardo.	
Data: 	2011-06-01	
Ano: 	2011	
Palavras-chave: 	Polyphasic Taxonomy; Β-tubulin gene; Aspergillus spp morphology.	
Resumo: 	In spite of the taxonomy of the Aspergillus species of the Nigri Section being regarded as troublesome, a number of methods have been proposed to aid in the classification of this Section. This work aimed to distinguish Aspergillus species of the Nigri Section from foods, grains and caves on the basis in Polyphasic Taxonomy by utilizing morphologic and physiologic characters, and sequencing of ß-tubulin and calmodulin genes. The morphologic identification proved useful for some species, such as A. carbonarius and Aspergillus sp UFLA DCA 01, despite not having been totally effective in elucidating species related to A. niger. The isolation of the species of the Nigri Section on Creatine Sucrose Agar (CREA) enabled to distinguish the Aspergillus sp species, which was characterized by the lack of sporulation and by the production of sclerotia. Scanning Electron microscopy (SEM) allowed distinguishing the species into two distinct groups. The production of Ochratoxin A (OTA) was only found in the A. carbonarius and A. niger species. The sequencing of β-tubulin gene was efficient in differing most of the Aspergillus species from the Nigri Section with the exception of Aspergillus UFLA DCA 01, which could not be distinguished from A. costaricaensis. This species is morphologically similar to A. costaricaencis for its low sporulation capacity and high sclerotia production, but it differs morphologically from A. costaricaensis for its conidial ornamentation and size of vesicles. Equally, based on partial calmodulin gene sequence data Aspergillus UFLA DCA 01 differs from A. costaricaensis.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200044	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822011000200044	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.42 n.2 2011	
Fonte: 	info:eu-repo/semantics/openAccess