Data/hora: 06/06/2024 01:04:18

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction	
Autores: 	Barouni,Abdulmonam Saied; Saridakis,Halha Ostrensky; Vidotto,Marilda Carlos.	
Data: 	2004-06-01	
Ano: 	2004	
Palavras-chave: 	Mycobacterium tuberculosis; Multiprimer-PCR; Molecular diagnosis.	
Resumo: 	We investigated the use of multiprimer-PCR for detection of mycobacteria species in clinical samples. Three different mycobacterial genomic fragments were investigated: the IS6110 insertion sequence, present in M. tuberculosis complex; the genus specific fragment (32kDa); and from M. tuberculosis species-specific mtp40 gene. The sensitivity and specificity using 135 clinical isolates were 94.5% and 95.9%, respectively, compared with culture in Löwenstein-Jensen medium; the detection limit was 0.05ng of DNA. In conclusion, this assay is reliable and rapid for detection of Mycobacterium species in clinical samples, and differentiates M. tuberculosis from M. bovis strains in a single-step assay.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100004	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822004000100004	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.35 n.1-2 2004	
Fonte: 	info:eu-repo/semantics/openAccess