Data/hora: 13/06/2024 09:37:54

Provedor de dados: 	58	
País: 	Brazil	
Título: 	The virulence of Streptococcus pneumoniae partially depends on dprA	
Autores: 	Yu,Yi; Chang,De; Xu,Huiwen; Zhang,Xuelin; Pan,Lei; Xu,Chou; Huang,Bing; Zhou,Hong; Li,Jia; Guo,Jun; Liu,Changting.	
Data: 	2017-06-01	
Ano: 	2017	
Palavras-chave: 	Streptococcus pneumoniae; DprA; Transformation; Virulence.	
Resumo: 	Abstract Streptococcus pneumoniae is one of the most frequent opportunistic pathogens worldwide. DNA processing protein A (DprA) is an important factor involved in bacterial uptake and DNA integration into bacterial genome, but its role in S. pneumoniae virulence remains unclear. The aim of this study was to characterize the effects of the pneumococcal dprA gene on the pathogenesis of S. pneumoniae. To construct a dprA-deficient pneumococcal strain, the dprA gene of the S. pneumoniae strain D39 was inactivated. The virulence of this dprA-deficient strain, designated ΔD39, was compared with that of the wild-type strain by evaluating their respective capabilities to adhere to human pulmonary epithelial cells (PEC-A549) and by analyzing their choline-binding protein expression levels. In addition, the expression profiles of genes associated with virulence and host survival assays were also conducted with the mutant and the wild-type strain. Our results indicate that the capability of ΔD39 to adhere to the PEC-A549 airway cells was significantly lower (p &lt; 0.01) compared with D39. Additionally, the 100-KD choline-binding protein was not detected in ΔD39. The addition of competence-stimulating peptide (CSP) lead to a significantly reduction of psaA mRNA expression in the dprA-deficient mutant and an increased level of psaA transcripts in the wild-type strain (p &lt; 0.01). The median survival time of mice intraperitoneally infected with ΔD39 was significantly higher (p &lt; 0.01) than that of mice infected with D39. The results of this study suggest that DprA has a significant effect on virulence characteristics of S. pneumoniae by influencing the expression of choline-binding protein and PsaA.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200225	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2016.10.019	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.48 n.2 2017	
Fonte: 	info:eu-repo/semantics/openAccess