Data/hora: 10/06/2024 00:50:08

Provedor de dados: | 58
País: | Brazil
Título: | Production of Brazilian human norovirus VLPs and comparison of purification methods
Autores: | Lamounier,Thais Alves da Costa; Oliveira,Layssa Miranda de; Camargo,Brenda Rabello de; Rodrigues,Kelly Barreto; Noronha,Eliane Ferreira; Ribeiro,Bergmann Morais; Nagata,Tatsuya.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Norwalk virus; VLP; Ion-exchange chromatography.
Resumo: | Abstract Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-838246420140925
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.46 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess