Data/hora: 02/06/2024 08:46:47

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Concomitant production of two proteases and alpha-amylase by a novel strain of Bacillus subtilis in a microprocessor controlled bioreactor	
Autores: 	Mukhtar,Hamid; Ikram-ul-Haq,.	
Data: 	2012-09-01	
Ano: 	2012	
Palavras-chave: 	Simultaneous; Sporulation; Kinetics; Fermentation; Agitation.	
Resumo: 	We describe the simultaneous production of Bacillus subtilis based proteases and alpha amylase using a computer controlled laboratory scale 7.5 L batch bioreactor. The present strain is the first to be reported that concomitantly produces these two industrially important enzymes. The growth and sporulation of Bacillus subtilis was monitored and maximum production of alkaline protease and alpha amylase was found to coincide with maximum sporulation. Two types of proteases were detected in the fermentation broth; a neutral and an alkaline protease most active in a pH range of 7.0-8.0 and 8.0-10, respectively. Maximum production of proteases was observed at an incubation temperature of 37ºC while that of alpha amylase was observed at 40ºC. The optimum aeration and agitation levels for protease production were 0.6 L/L/min and 200rpm, respectively, and for alpha amylase were 0.6 L/L/min and 150 rpm. The kinetic parameters Yp/x and qp were also found to be significant at the given fermentation conditions.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300033	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822012000300033	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.43 n.3 2012	
Fonte: 	info:eu-repo/semantics/openAccess