Data/hora: 12/06/2024 14:01:44

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Characterization of Klebsiella pneumoniae associated with cattle infections in southwest China using multi-locus sequence typing (MLST), antibiotic resistance and virulence-associated gene profile analysis
Autores: | Cheng,Fangjun; Li,Zhangcheng; Lan,Shimei; Liu,Wei; Li,Xiaoyan; Zhou,Zuoyong; Song,Zhenhui; Wu,Juan; Zhang,Manli; Shan,Wenjie.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Cattle; Antimicrobial susceptibility; Klebsiella pneumonia; MLST; Virulence genes.
Resumo: | Abstract Klebsiella pneumoniae is important human and animal pathogen that causes a wide spectrum of infections. In this study, isolates from cattle nasal swabs samples were identified by 16S rRNA, and to evaluate the antimicrobial susceptibility, virulence gene carrying levels, and multilocus sequence typing of K. pneumoniae isolates. 33 isolates of K. pneumoniae were isolated and identified in 213 nasal swabs samples, of which 12 were hypervirulent K. pneumoniae strains. Extended Spectrum Beta-Lactamases genes were found in 93.4% of the strains. Of which, TEM was the most prevalent (93.4%), followed by CTX-M and SHV were 57.6% and 39.4%, respectively. A main mutation pattern of quinoloneresistance-determining region, Thr83-Ieu and Asp87-Asn in gyrA and Ser87-Ile in parC, was detected in 33 K. pneumoniae isolates. All the isolates harbored at least two virulence factor genes, with ureA (97.0%) and wabG (91.0%) exhibiting high carriage rates in 33 K. pneumoniae isolates. MLST revealed 7 sequence types, of which 3 STs (2541, 2581 and 2844) were newly assigned. Using eBURST, ST2844 and ST2541 were assigned to new clonal complex 2844. Our study provides evidence and biological characteristics of K. pneumoniae isolates from cattle upper respiratory tract in Southwest China.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500093
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2018.06.004
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.49 suppl.1 2018
Fonte: | info:eu-repo/semantics/openAccess