Data/hora: 13/06/2024 15:11:09

Provedor de dados: | 58
País: | Brazil
Título: | Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties
Autores: | Guedes,Helma V.; Santos,Samuel T. dos; Perin,Liamara; Teixeira,Kátia R. dos S.; Reis,Veronica M.; Baldani,José I.
Data: | 2008-12-01
Ano: | 2008
Palavras-chave: | Horizontal spread; Resistance plasmids; ESBL.
Resumo: | A polyphasic approach was applied to characterize 35 G. diazotrophicus isolates obtained from sugarcane varieties cultivated in Brazil. The isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ARDRA and RISARFLP techniques). Variability among the isolates was observed in relation to the carbon source use preference. Glucose and sucrose were used by all isolates in contrast to myo-inositol, galactose and ribose that were not metabolized. The results of the analysis showed the presence of two groups clustered at 68% of similarity. The genetic distance was higher when RISA-RFLP analysis was used. Analysis of 16S rDNA sequences from isolates showed that all of them belonged to the G. diazotrophicus species. Neither effect of the plant part nor sugarcane variety was observed during the cluster analysis. The observed metabolic and genetic variability will be helpful during the strain selection studies for sugarcane inoculation in association with sugarcane breeding programs.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400023
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822008000400023
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.39 n.4 2008
Fonte: | info:eu-repo/semantics/openAccess