Data/hora: 03/06/2024 16:37:38

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Caliciviruses in hospitalized children, São Luís, Maranhão, 1997-1999: detection of norovirus GII.12	
Autores: 	Portal,Thayara Morais; Siqueira,Jones Anderson Monteiro; Costa,Larissa Cristina Prado das Neves; Lima,Ian Carlos Gomes de; Lucena,Maria Silvia Sousa de; Bandeira,Renato da Silva; Linhares,Alexandre da Costa; Luz,Claudia Regina Nunes Eloi da; Gabbay,Yvone Benchimol; Resque,Hugo Reis.	
Data: 	2016-09-01	
Ano: 	2016	
Palavras-chave: 	Norovirus; Sapovirus; Gastroenteritis; Children.	
Resumo: 	ABSTRACT Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6% (24/136) and 32.6% (15/46), respectively in diarrheic patients and 10.0% (9/90) in non-diarrheic patients (p &lt; 0.01). Of the diarrheic patients, 17% had fever, vomiting and anorexia, and 13% developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50% (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8% (11/112), with 22.6% (7/31) in diarrheic patients and 4.9% (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3% had fever, vomiting and anorexia, whereas 18.2% had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300724	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2016.04.008	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.47 n.3 2016	
Fonte: 	info:eu-repo/semantics/openAccess