Data/hora: 16/06/2024 16:50:56

Provedor de dados: | 58
País: | Brazil
Título: | High frequency of hepatitis E virus infection in swine from South Brazil and close similarity to human HEV isolates
Autores: | Passos-Castilho,Ana Maria; Granato,Celso Francisco Hernandes.
Data: | 2017-06-01
Ano: | 2017
Palavras-chave: | Brazil; Genotype 3; Hepatitis E virus; Pigs; Zoonosis.
Resumo: | Abstract Hepatitis E virus is responsible for acute and chronic liver infections worldwide. Swine hepatitis E virus has been isolated in Brazil, and a probable zoonotic transmission has been described, although data are still scarce. The aim of this study was to investigate the frequency of hepatitis E virus infection in pigs from a small-scale farm in the rural area of Paraná State, South Brazil. Fecal samples were collected from 170 pigs and screened for hepatitis E virus RNA using a duplex real-time RT-PCR targeting a highly conserved 70 nt long sequence within overlapping parts of ORF2 and ORF3 as well as a 113 nt sequence of ORF2. Positive samples with high viral loads were subjected to direct sequencing and phylogenetic analysis. hepatitis E virus RNA was detected in 34 (20.0%) of the 170 pigs following positive results in at least one set of screening real-time RT-PCR primers and probes. The swine hepatitis E virus strains clustered with the genotype hepatitis E virus-3b reference sequences in the phylogenetic analysis and showed close similarity to human hepatitis E virus isolates previously reported in Brazil.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200373
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2016.10.022
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.48 n.2 2017
Fonte: | info:eu-repo/semantics/openAccess