Data/hora: 10/06/2024 17:23:04

Provedor de dados: | 58
País: | Brazil
Título: | Evaluation of the counterimmunoelectrophoresis reaction as a serodiagnosis test for swine leptospirosis
Autores: | Genovez,Margareth Élide; Yasuda,Paulo Hideki; Vasconcellos,Silvio Arruda; Scarcelli,Eliana; Cardoso,Maristela Vasconcellos; Girio,Raul da Silva.
Data: | 2001-06-01
Ano: | 2001
Palavras-chave: | Counterimmunoelectrophoresis; Swine leptospirosis; Seradiagnosis.
Resumo: | The Counterimmunoelectrophoresis reaction (CIE) was tested as a genus specific test for swine leptospirosis diagnosis using three soluble extracts obtained from Leptospira sp, serovars pomona, icterohaemorrhagiae and patoc. The extracts were treated with hot Triton X-100 and applied to serum samples of animals divided in three groups: Group 1, 10 swines experimentally infected with the Pomona strain; Group 2, 50 naturally infected swines and Group 3, 10 swines control. These animals were serologically evaluated by CIE and by the Microscopic Agglutination Test (MAT), the WHO reference technique. Groups 1 and 3 were monitored during a period of 93 days after infection (a.i.). Group 1 serum convertion took place around the 10th day a.i. by MAT but ocurred earlier by CIE using any antigen. When CIE was carried out with the homologous antigen to the experimental infection, the results were consistent with MAT, but not when the heterologous antigens were used. Groups 1 and 3 showed distinct results: in Group 3, diferences between results of CIE accomplished with any three antigen extracts were not significant, indicating lack of dependence on the serovar responsible for the outbreak. Although being safe, fast, easy to perform, inexpensive and ideal for analysis of large number of samples, CIE revealed limited genus specificity, which is not convenient for field screening tests. The advantage of CIE is the capability to detect antibodies earlier than MAT.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200016
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822001000200016
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.32 n.2 2001
Fonte: | info:eu-repo/semantics/openAccess