Data/hora: 03/06/2024 18:08:21

Provedor de dados: | 58
País: | Brazil
Título: | Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance
Autores: | Reis,Vanda Renata; Antonangelo,Ana Teresa Burlamaqui Faraco; Bassi,Ana Paula Guarnieri; Colombi,Débora; Ceccato-Antonini,Sandra Regina.
Data: | 2017-06-01
Ano: | 2017
Palavras-chave: | Fermentation; Stresses; Yeasts; Microsatellite; Contaminants.
Resumo: | Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cerevisiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200268
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2016.09.017
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.48 n.2 2017
Fonte: | info:eu-repo/semantics/openAccess