Data/hora: 09/06/2024 04:40:00

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Action of phosphorylated mannanoligosaccharides on immune and hematological responses and fecal consistency of dogs experimentally infected with enteropathogenic Escherichia coli strains
Autores: | Gouveia,E.M.M.F.; Silva,I.S.; Nakazato,G.; Onselem,V.J.V.; Corrêa,R.A.C.; Araujo,F.R.; Chang,M.R.
Data: | 2013-01-01
Ano: | 2013
Palavras-chave: | Enteropathogenic E. coli; In vivo experiment; Dogs; Phosphorylated 31 mannanoligo saccharides; PCR.
Resumo: | The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E. coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to number of blood cells in the hemogram and IgA and IgG production. MOS was effective in recovering of EPEC-infected dogs since prebiotic-treated animals recovered more rapidly from infection than untreated ones (p &lt; 0.05). This is an important finding since diarrhea causes intense dehydration and nutrient loss. The use of prebiotics for humans and other animals with diarrhea can be an alternative for the treatment and prophylaxis of EPEC infections.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200027
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822013000200027
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.44 n.2 2013
Fonte: | info:eu-repo/semantics/openAccess