Data/hora: 02/06/2024 12:55:58

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis
Autores: | Ciftci,Alper; Findik,Arzu; Onuk,Ertan Emek; Savasan,Serap.
Data: | 2009-06-01
Ano: | 2009
Palavras-chave: | Staphylococcus aureus; IcaA; IcaD; Slime; Mastitis.
Resumo: | This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains were positive for icaA and icaD gene, respectively. Only 2 of 59 strains were positive for both methicillin resistance and slime producing, phenotypically, suggesting lack of correlation between methicillin resistance and slime production in these isolates. In conclusion, the optimized triplex PCR in this study was useful for rapid and reliable detection of methicillin resistant S. aureus. Furthermore, only PCR targetting icaA and icaD may not sufficient to detect slime production and further studies targetting other ica genes should be conducted for accurate evaluation of slime production characters of S. aureus strains.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200009
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822009000200009
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.40 n.2 2009
Fonte: | info:eu-repo/semantics/openAccess