Data/hora: 04/06/2024 09:37:11

Provedor de dados: 	58	
País: 	Brazil	
Título: 	A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour	
Autores: 	Coelho,Sheila Lorena de Araújo; Magalhães,Valter Cruz; Marbach,Phellippe Arthur Santos; Cazetta,Marcia Luciana.	
Data: 	2016-03-01	
Ano: 	2016	
Palavras-chave: 	Microbial enzyme; Fermentation; Agro-industrial substrates.	
Resumo: 	Abstract Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars, cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for β-CGTase production by Bacillus sp. SM-02, isolated from soil of cassava industries waste water lake. The optimization was performed by Central Composite Design (CCD) 2, using cassava flour and corn steep liquor as substrates. The maximum production of 1087.9 U mL−1 was obtained with 25.0 g L−1 of cassava flour and 3.5 g L−1 of corn steep after 72 h by submerged fermentation. The enzyme showed optimum activity at pH 5.0 and temperature 55 °C, and maintained thermal stability at 55 °C for 3 h. The enzymatic activity was stimulated in the presence of Mg+2, Ca+2, EDTA, K+, Ba+2 and Na+ and inhibited in the presence of Hg+2, Cu+2, Fe+2 and Zn+2. The results showed that Bacillus sp. SM-02 have good potential for β-CGTase production.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100120	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2015.11.018	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.47 n.1 2016	
Fonte: 	info:eu-repo/semantics/openAccess