Data/hora: 04/06/2024 02:26:07

Provedor de dados: 	Brazilian Journal of Microbiology	
País: 	Brazil	
Título: 	Diagnosis of the pulmonary tuberculosis by polymerase chain reaction: a comparative study between HIV - positive and - negative individuals	
Autores: 	Silva,Rosemeri Maurici da; Machado,Tairone; Bazzo,Maria Luiza.	
Data: 	2012-03-01	
Ano: 	2012	
Palavras-chave: 	Tuberculosis; HIV; PCR; Sputum.	
Resumo: 	This study was performed to assess the efficiency of polymerase chain reaction (PCR) directly from sputum for the diagnosis of pulmonary tuberculosis by comparison between HIV-positive and HIV-negative individuals. Sputum samples were collected from hospitalized patients admitted with a clinical diagnosis of pulmonary tuberculosis, and subjected to smear microscopy, culture on LJ medium and detection of M. tuberculosis by PCR. Sensitivity, specificity, and predictive values (positive and negative) were calculated using smear and/or culture at day 42 as the gold standard, by comparing the yield in HIV-positive and HIV-negative individuals. Regardless of serostatus, the technique's yield had 62% sensitivity, 70% specificity, 79% positive predictive value, 50% negative predictive value, and 65% accuracy. HIV-negative had 64% sensitivity, 74% specificity, 75% positive predictive value, 63% negative predictive value, and 68% accuracy. HIV-positive had 59% sensitivity, 33% specificity, 87% positive predictive value, 10% negative predictive value, and 56% accuracy. The PCR showed a higher yield in HIV-negative individuals compared to HIV-positive individuals.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100030	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822012000100030	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.43 n.1 2012	
Fonte: 	info:eu-repo/semantics/openAccess