Data/hora: 09/06/2024 14:50:47

Provedor de dados: 	Brazilian Journal of Microbiology	
País: 	Brazil	
Título: 	Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee	
Autores: 	Tajabadi,Naser; Mardan,Makhdzir; Saari,Nazamid; Mustafa,Shuhaimi; Bahreini,Rasoul; Manap,Mohd Yazid Abdul.	
Data: 	2013-09-01	
Ano: 	2013	
Palavras-chave: 	Honey bee; Honey stomach; Lactobacillus; Probiotic; 16S rDNA gene sequences.	
Resumo: 	This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to Lactobacillus pentosus. However, only one phylotype was found to be distantly linked to the Lactobacillus fermentum. The outcomes of the present study indicated that L. plantarum, L. pentosus, and L. fermentum were the dominant lactobacilli in the honey stomach of honeybee A. dorsata collected during the dry season from Malaysia forest area - specifically "Melaleuca in Terengganu".	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300008	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822013000300008	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.44 n.3 2013	
Fonte: 	info:eu-repo/semantics/openAccess