Data/hora: 03/06/2024 16:52:57

Provedor de dados: | 58
País: | Brazil
Título: | Microbial mediated preparation, characterization and optimization of gold nanoparticles
Autores: | Barabadi,Hamed; Honary,Soheila; Ebrahimi,Pouneh; Mohammadi,Milad Ali; Alizadeh,Ahad; Naghibi,Farzaneh.
Data: | 2014-12-01
Ano: | 2014
Palavras-chave: | Biosynthesis; Optimization; Gold nanoparticles; Box-behnken design; Penicillium crustosum.
Resumo: | The need for eco-friendly and cost effective methods for nanoparticles synthesis is developing interest in biological approaches which are free from the use of toxic chemicals as byproducts. This study aimed to biosynthesize and optimize the size of gold nanoparticles which produced by biotechnological method using Penicillium crustosum isolated from soil. Initially, Penicillium crustosum was grown in fluid czapek dox broth on shaker at 28 ºC and 200 rpm for ten days and then the supernatant was separated from the mycelia to convert AuCl4 solution into gold nanoparticles. The synthesized nanoparticles in the optimum conditions were formed with fairly well-defined dimensions and good monodispersity. The characterizations were done by using different methods (UV-Visible Spectroscopy, Fluorescence, FT-IR, AFM (Atomic Force Microscopy) and DLS (Dynamic Light Scattering). The bioconversion was optimized by Box-Behnken experimental design. The results show that the effective factors in this process were concentration of AuCl4, pH of medium and temperature of shaker incubator. The R² value was calculated to be 0.9999 indicating the accuracy and ability of the polynomial model. It can be concluded that the use of multivariate analysis facilitated to find out the optimum conditions for the biosynthesis of gold nanoparticles induced by Penicillium crustosum in a time and cost effective process. The current approach suggested that rapid synthesis of gold nanoparticles would be suitable for developing a biological process for mass scale production of formulations.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400046
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822014000400046
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.45 n.4 2014
Fonte: | info:eu-repo/semantics/openAccess