Data/hora: 14/06/2024 00:14:26

Provedor de dados: | 58
País: | Brazil
Título: | Transcription of interferon stimulated genes in response to porcine rubulavirus infection in vitro
Autores: | Flores-Ocelotl,María del Rosario; Rosas-Murrieta,Nora Hilda; Vallejo-Ruiz,Verónica; Reyes-Leyva,Julio; Herrera-Camacho,Irma; Santos-López,Gerardo.
Data: | 2011-09-01
Ano: | 2011
Palavras-chave: | Porcine rubulavirus; Interferon; STAT; LPMV; Gene expression.
Resumo: | Porcine rubulavirus (PoRV) is an emerging virus causing meningo-encephalitis and reproductive failures in pigs. Little is known about the pathogenesis and immune evasion of this virus; therefore research on the mechanisms underlying tissue damage during infection is essential. To explore these mechanisms, the effect of PoRV on the transcription of interferon (IFN) pathway members was analyzed in vitro by semi-quantitative RT-PCR. Ten TCID50 of PoRV stimulated transcription of IFNα, IFNβ, STAT1, STAT2, p48 and OAS genes in neuroblastoma cells, whereas infection with 100 TCID50 did not stimulate transcription levels more than non-infected cells. When the cells were primed with IFNα, infection with 1 TCDI50 of PoRV sufficed to stimulate the transcription of the same genes, but 10 and 100 TCID50 did not modify the transcription level of those genes as compared with non-infected and primed controls. MxA gene transcription was observed only when the cells were primed with IFNα and stimulated with 10 TCID50, whereas 100 TCID50 of PoRV did not modify the MxA transcription level as compared to non-infected and primed cells. Our results show that PoRV replication at low titers stimulates the expression of IFN-responsive genes in neuroblastoma cells, and suggest that replication of PoRV at higher titers inhibits the transcription of several members of the IFN pathway. These findings may contribute to the understanding of the pathogenesis of PoRV.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300041
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822011000300041
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.42 n.3 2011
Fonte: | info:eu-repo/semantics/openAccess