Data/hora: 29/05/2024 02:21:37

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Agro-industrial residues and starch for growth and co-production of polyhydroxyalkanoate copolymer and α-amylase by Bacillus sp. CFR-67
Autores: | Shamala,T. R.; Vijayendra,S.V.N.; Joshi,G.J.
Data: | 2012-09-01
Ano: | 2012
Palavras-chave: | Α-amylase; Bacillus sp.; Corn starch; Polyhydroxyalkanoate; Rice bran hydrolysate; Wheat bran hydrolysate.
Resumo: | Polyhydroxyalkanoates (PHA) and α-amylase (α-1,4 glucan-4-glucanohydrolase, E.C. 3.2.1.1) were co-produced by Bacillus sp. CFR-67 using unhydrolysed corn starch as a substrate. Bacterial growth and polymer production were enhanced with the supplementation of hydrolysates of wheat bran (WBH) or rice bran (RBH) individually or in combination (5-20 g L-1, based on weight of soluble substrates-SS). In batch cultivation, a mixture of WBH and RBH (1:1, 10 g L-1 of SS) along with ammonium acetate (1.75 g L-1) and corn starch (30 g L-1) produced maximum quantity of biomass (10 g L-1) and PHA (5.9 g L-1). The polymer thus produced was a copolymer of polyhydroxybutyrate-co-hydroxyvalerate of 95:5 to 90:10 mol%. Presence of WBH and corn starch (10-50 g L-1) in the medium enhanced fermentative yield of α-amylase (2-40 U mL-1 min-1). The enzyme was active in a wide range of pH (4-9) and temperature (40-60ºC). This is the first report on simultaneous production of copolymer of bacterial PHA and α-amylase from unhydrolysed corn starch and agro-industrial residues as substrates.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300036
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822012000300036
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.43 n.3 2012
Fonte: | info:eu-repo/semantics/openAccess