Data/hora: 02/06/2024 13:07:22

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
Autores: | Alam,Mohammad Zubair; Aqil,Farrukh; Ahmad,Iqbal; Ahmad,Shamim.
Data: | 2013-09-01
Ano: | 2013
Palavras-chave: | Beta-lactamase; ESβL; Antibiotic resistance; Enteric bacteria; Plasmid transfer.
Resumo: | This study reports the occurrence of antibiotic resistance and production of β-lactamases including extended spectrum beta-lactamases (ESβL) in enteric bacteria isolated from hospital wastewater. Among sixty-nine isolates, tested for antibiotic sensitivity, 73.9% strains were resistant to ampicillin followed by nalidixic acid (72.5%), penicillin (63.8%), co-trimoxazole (55.1%), norfloxacin (53.6%), methicillin (52.7%), cefuroxime (39.1%), cefotaxime (23.2%) and cefixime (20.3%). Resistance to streptomycin, chloramphenicol, nitrofurantoin, tetracycline, and doxycycline was recorded in less than 13% of the strains. The minimum inhibitory concentration (MIC) showed a high level of resistance (800-1600 µg/mL) to one or more antibiotics. Sixty three (91%) isolates produced β-lactamases as determined by rapid iodometric test. Multiple antibiotic resistances were noted in both among ESβL and non-ESβL producers. The β-lactamases hydrolyzed multiple substrates including penicillin (78.8% isolates), ampicillin (62.3%), cefodroxil (52.2%), cefotoxime (21.7%) and cefuroxime (18.8%). Fifteen isolates producing ESβLs were found multidrug resistant. Four ESβL producing isolates could transfer their R-plasmid to the recipient strain E. coli K-12 with conjugation frequency ranging from 7.0 x 10-3 to 8.8 x 10-4. The findings indicated that ESβL producing enteric bacteria are common in the waste water. Such isolates may disseminate the multiple antibiotic resistance traits among bacterial community through genetic exchange mechanisms and thus requires immediate attention.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300021
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822013000300021
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.44 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess