Data/hora: 09/06/2024 15:38:09

Provedor de dados: | 58
País: | Brazil
Título: | Polysaccharide production in batch process of Neisseria meningitidis serogroup C comparing Frantz, modified Frantz and Cartlin 6 cultivation media
Autores: | Paz,Marcelo Fossa da; Baruque-Ramos,Júlia; Hiss,Haroldo; Vicentin,Márcio Alberto; Leal,Maria Betania Batista; Raw,Isaías.
Data: | 2003-04-01
Ano: | 2003
Palavras-chave: | Neisseria meningitidis; Culture medium; Vaccine; Polysaccharide; Batch cultivation.
Resumo: | Polysaccharide of N. meningitidis serogroup C constitutes the antigen for the vaccine against meningitis. The goal of this work was to compare three cultivation media for production of this polysaccharide: Frantz, modified Frantz medium (with replacement of glucose by glycerol), and Catlin 6 (a synthetic medium with glucose). The comparative criteria were based on the final polysaccharide concentrations and the yield coefficient cell/polysaccharide (Y P/X). The kinetic parameters: pH, substrate consumption and cell growth were also determined. For this purpose, 9 cultivation runs were carried out in a 80 L New Brunswick bioreactor, under the following conditions: 42 L of culture medium, temperature 35ºC, air flow 5 L/min, agitation frequency 120 rpm and vessel pressure 6 psi, without dissolved oxygen or pH controls. The cultivation runs were divided in three groups, with 3 repetitions each. The cultivation using the Frantz medium presented the best results: average of final polysaccharide concentration = 0.134 g/L and Y P/X=0.121, followed by Catlin 6 medium, with results of 0.095 g/L and 0.067 respectively. Considering the principal advantages in the use of the synthetic medium, i.e. facilitation of a cultivation and purification steps of the polysaccharide production process, there is a possibility that in the near future, Catlin 6 will replace the traditional Frantz medium.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100007
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822003000100007
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.34 n.1 2003
Fonte: | info:eu-repo/semantics/openAccess