Data/hora: 10/06/2024 02:13:41

Provedor de dados: 	58	
País: 	Brazil	
Título: 	KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil	
Autores: 	Biberg,Camila Arguelo; Rodrigues,Ana Claudia Souza; Carmo,Sidiane Ferreira do; Chaves,Claudia Elizabeth Volpe; Gales,Ana Cristina; Chang,Marilene Rodrigues.	
Data: 	2015-06-01	
Ano: 	2015	
Palavras-chave: 	Klebsiella pneumoniae; Carbapenems; Drug-resistant bacteria.	
Resumo: 	The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-838246246220140174	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.46 n.2 2015	
Fonte: 	info:eu-repo/semantics/openAccess