Data/hora: 10/06/2024 02:48:00

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Protoplast formation and regeneration from Streptomyces clavuligerus NRRL 3585 and clavulanic acid production	
Autores: 	Carneiro-da-Cunha,Maria das Graças; Lima Filho,José Luiz de; Campos-Takaki,Galba Maria de.	
Data: 	2002-12-01	
Ano: 	2002	
Palavras-chave: 	Streptomyces clavuligerus; Protoplast; Clavulanic acid.	
Resumo: 	Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC-3.2.1.17) 40,000 U (1mg/mL), in an osmotic solution for 90 min at 28ºC. The frequency of regenerated protoplasts in the lag phase was 1.7x10³ CFU/mL (28.97%), in the beginning of the exponential phase 0.4x10² CFU/mL (31.67%), in the exponential growth phase 2.5x10³ CFU/mL (46.30%) and 1.0x10(5) CFU/mL in stationary phase (48.45%). Antibiotic production and activity of regenerated protoplasts were observed in all phases, except in the lag phase. The protoplast formation and regeneration techniques resulted in a new isolate strain of Streptomyces clavuligerus that produced approximately 2.5 fold more clavulanic acid.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400014	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822002000400014	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.33 n.4 2002	
Fonte: 	info:eu-repo/semantics/openAccess