Data/hora: 09/06/2024 13:55:18

Provedor de dados: | 58
País: | Brazil
Título: | Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol
Autores: | Rana,Inder Singh; Rana,Aarti Singh; Rajak,Ram Charan.
Data: | 2011-12-01
Ano: | 2011
Palavras-chave: | Essential oil; Syzygium aromaticum; Antifungal; Chromatography; Eugenol.
Resumo: | Antifungal properties of some essential oils have been well documented. Clove oil is reported to have strong antifungal activity against many fungal species. In this study we have evaluated antifungal potential of essential oil of Syzygium aromaticum (L.) against some common fungal pathogens of plants and animals namely, Fusarium moniliforme NCIM 1100, Fusarium oxysporum MTCC 284, Aspergillus sp., Mucor sp., Trichophyton rubrum and Microsporum gypseum. All fungal species were found to be inhibited by the oil when tested through agar well diffusion method. Minimum inhibitory concentration (MIC) was determined for all the species. Column chromatography was performed to separate the eugenol rich fraction from clove oil. Out of seven fractions maximum activity was obtained in column fraction II. TLC and HPLC data confirmed presence of considerable Eugenol in fraction II and clove oil. Microscopic study on effect of clove oil and column fraction II on spores of Mucor sp. and M. gypseum showed distortion and shrinkage while it was absent in other column fractions. So it can be concluded that the antifungal action of clove oil is due to its high eugenol content.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400004
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822011000400004
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.42 n.4 2011
Fonte: | info:eu-repo/semantics/openAccess