Data/hora: 02/06/2024 15:52:35

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis	
Autores: 	Marques,Viviane Figueira; Motta,Cássia Couto da; Soares,Bianca da Silva; Melo,Dayanne Araújo de; Coelho,Shana de Mattos de Oliveira; Coelho,Irene da Silva; Barbosa,Helene Santos; Souza,Miliane Moreira Soares de.	
Data: 	2017-03-01	
Ano: 	2017	
Palavras-chave: 	Biofilm; Agr types; Antimicrobial resistance; Mastitis.	
Resumo: 	Abstract Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation. Biofilm production in microplates was evaluated considering the intervals determined along the bacterial growth curve. In addition, to determine the most suitable time interval for biofilm analysis, scanning electron microscopy was performed. Furthermore, genes such as mecA and blaZ that are related to beta-lactamic resistance and oxacillin susceptibility were tested. All the studied isolates were biofilm producers and mostly presented icaA and icaD. The Agr type II genes were significantly prevalent. According to the SEM, gradual changes in the bacterial arrangement were observed during biofilm formation along the growth curve phases, and the peak was reached at the stationary phase. In this study, the penicillin resistance was related to the production of beta-lactamase, and the high minimal bactericidal concentration for cefoxitin was possibly associated with biofilm protection. Therefore, further studies are warranted to better understand biofilm formation, possibly contributing to our knowledge about bacterial resistance in vivo.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100118	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2016.10.001	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.48 n.1 2017	
Fonte: 	info:eu-repo/semantics/openAccess