Data/hora: 09/06/2024 05:34:03

Provedor de dados: | 58
País: | Brazil
Título: | Comparison of electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni isolated from different animal species
Autores: | Scarcelli,Eliana; Costa,Elizabeth Oliveira da; Genovez,Margareth Élide; Cardoso,Maristela Vasconcellos; Bach,Erna Elizabeth; Torres,Ana Paula.
Data: | 2001-12-01
Ano: | 2001
Palavras-chave: | Campylobacter jejuni; SDS-PAGE; Biotyping.
Resumo: | Electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni strains isolated from feces of seven animal species, including man, were compared. Fourteen strains (two from each species) plus two human strains and the reference one, were ruptured by ultrasound and their total soluble proteins were analyzed by SDS-PAGE technique in a 12% polyacrylamide gel with computerized densitometric reading by the molecular analyst software. All the strains had bands in common that correspond to 45 and 66 Kda molecular weight. The disagreement corresponded to a 97 to 200 Kda molecular weight region. From the 17 strains, 13 (76.5%), were classified as biotype I, three (17.6%) as biotype II and one (5.8%) as biotype III. Since protein extracts were obtained from cells grown under identical conditions, and thus, able to express the same phenotype, this disagreement region could be related to different genotypes or serotypes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400006
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822001000400006
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.32 n.4 2001
Fonte: | info:eu-repo/semantics/openAccess