Data/hora: 13/06/2024 07:15:04

Provedor de dados: | 58
País: | Brazil
Título: | LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
Autores: | Trangoni,Marcos D.; Gioffré,Andrea K.; Cerón Cucchi,María E.; Caimi,Karina C.; Ruybal,Paula; Zumárraga,Martín J.; Cravero,Silvio L.
Data: | 2015-06-01
Ano: | 2015
Palavras-chave: | Loop-mediated isothermal amplification; Molecular typing; Brucellosis; Paratuberculosis.
Resumo: | In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols were suitable for specifically identifying the respective pathogens; in the case of Brucella, it also allowed the identification of all the biovars tested. We conclude that LAMP is a suitable rapid molecular typing tool that could help to shorten the time required to identify insidious bacteria in low-complexity laboratories, mainly in developing countries.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200619
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-838246220131206
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.46 n.2 2015
Fonte: | info:eu-repo/semantics/openAccess