Data/hora: 13/06/2024 01:37:13

Provedor de dados: 	58	
País: 	Brazil	
Título: 	LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis	
Autores: 	Trangoni,Marcos D.; Gioffré,Andrea K.; Cerón Cucchi,María E.; Caimi,Karina C.; Ruybal,Paula; Zumárraga,Martín J.; Cravero,Silvio L.	
Data: 	2015-06-01	
Ano: 	2015	
Palavras-chave: 	Loop-mediated isothermal amplification; Molecular typing; Brucellosis; Paratuberculosis.	
Resumo: 	In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols were suitable for specifically identifying the respective pathogens; in the case of Brucella, it also allowed the identification of all the biovars tested. We conclude that LAMP is a suitable rapid molecular typing tool that could help to shorten the time required to identify insidious bacteria in low-complexity laboratories, mainly in developing countries.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200619	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-838246220131206	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.46 n.2 2015	
Fonte: 	info:eu-repo/semantics/openAccess