Data/hora: 09/06/2024 12:31:50

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Chitinase Production by Streptomyces sp. ANU 6277
Autores: | Narayana,Kolla J.P.; Vijayalakshmi,Muvva.
Data: | 2009-12-01
Ano: | 2009
Palavras-chave: | Chitinase; Streptomyces sp. ANU 6277; Optimization; Characterization.
Resumo: | Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity was observed in the culture medium with pH 6 at 35ºC. Culture medium amended with 1% chitin was found to be suitable for maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as good carbon and nitrogen sources to enhance chitinase yield.Chitinase was purified from crude enzyme extract by single step gel filtration by Sephadex G-100. Purified chitinase of the strain exhibited a distinct protein band near 45 kDa by means of SDS-PAGE.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400002
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822009000400002
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.40 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess