Data/hora: 05/06/2024 09:26:19

Provedor de dados: | 58
País: | Brazil
Título: | High levels of catalase in sod mutants of Saccharomyces cerevisiae in high aeration conditions
Autores: | Martins,Vanessa; Manfredini,Vanusa; Benfato,Mara Silveira.
Data: | 2005-12-01
Ano: | 2005
Palavras-chave: | Catalase; Superoxide dismutase; High aeration; Hydrogen peroxide; S. cerevisiae.
Resumo: | Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1delta, sod2delta and sod1deltasod2delta mutants) in a stationary phase of growth under high aeration conditions were subjected to H2O2 stress. All the mutants were sensitive after H2O2 treatment. Glutathione peroxidase levels were significantly lower in sod1delta and sod2delta single mutants than in the wild-type without treatment. After exposure to H2O2 concentrations, glutathione peroxidase levels were increased in sod1deltasod2delta double mutants and the sod2delta single mutant, while sod1delta maintained lower gluthatione peroxidase activities. The sod2delta mutant demonstrated a similar catalase activity to that of the wild-type without treatment, whilst decreased catalase activity was observed in conditions of low aeration. Untreated sod1deltasod2delta double mutant cells presented a lower catalase activity. Catalase levels were higher under high aeration conditions than under microaerophilic conditions, including in sod1deltasod2delta cells that contain less H2O2, since SOD catalyzes the cleavage of superoxide producing H2O2 and oxygen. We suggest that catalase is not essential for sod mutants under normal conditions, but plays an important role in the acquisition of tolerance to oxidative stress induced by high aeration
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400008
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822005000400008
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.36 n.4 2005
Fonte: | info:eu-repo/semantics/openAccess