Data/hora: 07/06/2024 15:48:36

Provedor de dados: | 58
País: | Brazil
Título: | Characterization of a virulent Leptospira interrogans strain isolated from an abandoned swimming pool
Autores: | Forster,Karine M.; Hartwig,Daiane D.; Seixas,Fabiana K.; McBride,Alan J.A.; Monte,Leonardo G.; Recuero,Ana Lúcia C.; Brod,Claudiomar S.; Hartleben,Cláudia P.; Amaral,Marta; Dellagostin,Odir A.
Data: | 2013-01-01
Ano: | 2013
Palavras-chave: | Leptospira; Leptospirosis; Virulent; VNTR; RpoB.
Resumo: | Pathogenic Leptospira spp. are the etiological agents of leptospirosis, an important disease of both humans and animals. In urban settings, L. interrogans serovars are the predominant cause of disease in humans. The purpose of this study was to characterize a novel Leptospira isolate recovered from an abandoned swimming pool. Molecular characterization through sequencing of the rpoB gene revealed 100% identity with L. interrogans and variable-number tandem-repeat (VNTR) analysis resulted in a banding pattern identical to L. interrogans serogroup Icterohaemorrhagiae, serovar Copenhageni or Icterohaemorrhagiae. The virulence of the strain was determined in a hamster model of lethal leptospirosis. The lethal dose 50% (LD50) was calculated to be two leptospires in female hamsters and a histopathological examination of infected animals found typical lesions associated with severe leptospirosis, including renal epithelium degeneration, hepatic karyomegaly, liver-plate disarray and lymphocyte infiltration. This highly virulent strain is now available for use in further studies, especially evaluation of vaccine candidates.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100024
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822013005000029
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.44 n.1 2013
Fonte: | info:eu-repo/semantics/openAccess