Data/hora: 04/06/2024 03:39:43

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Electrical stimulation of saccharomyces cerevisiae cultures
Autores: | Araújo,Ofelia Q.F.; Coelho,Maria Alice Z.; Margarit,Isabel C.P.; Vaz-Junior,Carlos A.; Rocha-Leão,Maria Helena M.
Data: | 2004-06-01
Ano: | 2004
Palavras-chave: | Electrochemical stress; Saccharomyces cerevisiae; Cell cycle; Viability.
Resumo: | Modulation of cell endogenous membrane potential by an external electrical field influences the structure and function of membrane compartments, proteins and lipid bi-layer. In this work, the effects of applied potential on Saccharomyces cerevisiae growth were characterized through simple yet conclusive experiments. Cell growth time profile and cell division were investigated as macroscopic response to the electrical stimulation. Control experiments were conducted under identical conditions except for the absence of applied potential. Through comparative analysis, electrical stimulation was verified to alter cell cycle as smaller sized population was observed, suggesting that a synchrony in cell division was promoted. Power spectral analysis was employed to sustain synchrony enhancement, and mathematical modeling was conducted for determining kinetic growth changes. Monod type kinetic parameters for growth were determined by non-linear regression. The affinity constant (namely kS) presented a dependence on applied potential suggesting changes on transport across cell membrane. Electrochemically promoted stress was also verified to inhibit growth as well as to induce changes on cell viability.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100016
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822004000100016
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.35 n.1-2 2004
Fonte: | info:eu-repo/semantics/openAccess