Data/hora: 13/06/2024 08:19:11

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis
Autores: | Koga,Paula Célia Mariko; Menezes,Caroline Anunciação; Lima,Flávia Afonso; Nara,Júlia Mitico; Magalhães,Caroline Arantes; Cianciarullo,Aurora Marques; Ferreira-Júnior,Jorge Mario da Costa; Trabulsi,Luiz Rachid; Mendes-Ledesma,Meire Roberta Bresciani; Piazza,Roxane Maria Fontes.
Data: | 2003-11-01
Ano: | 2003
Palavras-chave: | Intimin; Phenotypical analysis; Polyclonal antibody; EPEC; EHEC.
Resumo: | Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822003000500002
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.34 suppl.1 2003
Fonte: | info:eu-repo/semantics/openAccess