Data/hora: 03/06/2024 00:54:37

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis	
Autores: 	Gibotti,Adriana; Tanaka,Tânia L.; Oliveira,Valéria R.; Taddei,Carla R.; Martinez,Marina B.	
Data: 	2004-06-01	
Ano: 	2004	
Palavras-chave: 	Ipa; Enteroinvasive Escherichia coli; Polymorphism; PCR-RFLP.	
Resumo: 	In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole structure, except in the carboxyl-terminal region.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822004000100012	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.35 n.1-2 2004	
Fonte: 	info:eu-repo/semantics/openAccess