Data/hora: 11/06/2024 01:06:52

Provedor de dados: 	Brazilian Journal of Microbiology	
País: 	Brazil	
Título: 	Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum	
Autores: 	Varavallo,Maurilio Antonio; Queiroz,Marisa Vieira de; Lana,Taís Guimarães; Brito,Admilson Toscano Ribeiro de; Gonçalves,Daniel Bonoto; Araújo,Elza Fernandes de.	
Data: 	2007-03-01	
Ano: 	2007	
Palavras-chave: 	Protoplast fusion; Pectinases; Penicillium.	
Resumo: 	Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P. expansum was induced by polyethylene glycol and calcium ions (Ca2+). Fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P. griseoroseum parental strain, presented a considerable increase in polygalacturonase (3-fold) and pectin lyase production (1.2-fold).	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100011	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822007000100011	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.38 n.1 2007	
Fonte: 	info:eu-repo/semantics/openAccess