Data/hora: 16/06/2024 06:27:09

Provedor de dados: | 58
País: | Brazil
Título: | Genetic variability of Brazilian isolates of Alternaria alternata detected by AFLP and RAPD techniques
Autores: | Dini-Andreote,Francisco; Pietrobon,Vivian Cristina; Andreote,Fernando Dini; Romão,Aline Silva; Spósito,Marcel Bellato; Araújo,Welington Luiz.
Data: | 2009-09-01
Ano: | 2009
Palavras-chave: | Alternaria brown spot; Molecular markers; Citrus plants; PCA analysis; Genetic variability.
Resumo: | The Alternaria brown spot (ABS) is a disease caused in tangerine plants and its hybrids by the fungus Alternaria alternata f. sp. citri which has been found in Brazil since 2001. Due to the recent occurrence in Brazilian orchards, the epidemiology and genetic variability of this pathogen is still an issue to be addressed. Here it is presented a survey about the genetic variability of this fungus by the characterization of twenty four pathogenic isolates of A. alternata f. sp. citri from citrus plants and four endophytic isolates from mango (one Alternaria tenuissima and three Alternaria arborescens). The application of two molecular markers Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) had revealed the isolates clustering in distinct groups when fingerprintings were analyzed by Principal Components Analysis (PCA). Despite the better assessment of the genetic variability through the AFLP, significant modifications in clusters components were not observed, and only slight shifts in the positioning of isolates LRS 39/3 and 25M were observed in PCA plots. Furthermore, in both analyses, only the isolates from lemon plants revealed to be clustered, differently from the absence of clustering for other hosts or plant tissues. Summarizing, both RAPD and AFLP analyses were both efficient to detect the genetic variability within the population of the pathogenic fungus Alternaria spp., supplying information on the genetic variability of this species as a basis for further studies aiming the disease control.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300032
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822009000300032
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.40 n.3 2009
Fonte: | info:eu-repo/semantics/openAccess