Data/hora: 16/06/2024 09:06:30

Provedor de dados: | 58
País: | Brazil
Título: | Different culture media applied to the study of Cryptococcus neoformans susceptibility to amphotericin B and fluconazole
Autores: | Alves,Sydney Hartz; Oliveira,Loiva T.; Goulart,Letícia S.; Linares,Carlos Eduardo B.; Griebeler,Josiane; Santurio,Janio M.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | Cryptococcus neoformans; Susceptibility; Antifungal agents.
Resumo: | This study compared the performance of three culture media used for the determination of minimal inhibitory concentration (MIC) of amphotericin B and fluconazole against 48 Cryptococcus neoformans strains. For amphotericin B the media employed were RPMI 1640 medium, as indicated by NCCLS as reference, Yeast Nitrogen Base (YNB) as proposed by Ghannoum et al. (YNB-1), Antibiotic medium 3 (AM3) indicated by others, and YNB as proposed by us (YNB-2). In YNB-1, amphotericin B showed broader MIC ranges, varying from 0.125 µg/ml to 1.0 µg/ml; in RPMI 1640 medium and YNB-2 the MICs were similar and in AM3 the MIC ranges were narrower. The same pattern was observed for MIC 90%. For fluconazole the media employed were RPMI 1640 and YNB-1. The MIC ranges were broader and elevated when YNB-1 was employed. MIC 50% were 2.0 µg/ml in RPMI 1640 medium and 4.0 µg/ml in YNB-1. Our results indicate that RPMI 1640 supplemented with 2% glucose allowed a good growth at 48h; YNB as recommended by Ghannoum et al. provided a broader MIC range, AM3 provided a narrow MIC range for amphotericin B, and YNB-2 was very similar to RPMI 1640 medium.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100005
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822002000100005
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.33 n.1 2002
Fonte: | info:eu-repo/semantics/openAccess