Data/hora: 09/06/2024 03:37:27

Provedor de dados: 	Brazilian Journal of Microbiology	
País: 	Brazil	
Título: 	Computation of interactive effects and optimization of process parameters for alkaline lipase production by mutant strain of Pseudomonas aeruginosa using response surface methodology	
Autores: 	Bisht,Deepali; Yadav,Santosh Kumar; Darmwal,Nandan Singh.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	Alkaline lipase; Box-Behnken design; Pseudomonas aeruginosa; Response surface methodology.	
Resumo: 	Alkaline lipase production by mutant strain of Pseudomonas aeruginosa MTCC 10,055 was optimized in shake flask batch fermentation using response surface methodology. An empirical model was developed through Box-Behnken experimental design to describe the relationship among tested variables (pH, temperature, castor oil, starch and triton-X-100). The second-order quadratic model determined the optimum conditions as castor oil, 1.77 mL.L-1; starch, 15.0 g.L-1; triton-X-100, 0.93 mL.L-1; incubation temperature, 34.12 ºC and pH 8.1 resulting into maximum alkaline lipase production (3142.57 U.mL-1). The quadratic model was in satisfactory adjustment with the experimental data as evidenced by a high coefficient of determination (R²) value (0.9987). The RSM facilitated the analysis and interpretation of experimental data to ascertain the optimum conditions of the variables for the process and recognized the contribution of individual variables to assess the response under optimal conditions. Hence Box-Behnken approach could fruitfully be applied for process optimization.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100035	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822013005000016	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.44 n.1 2013	
Fonte: 	info:eu-repo/semantics/openAccess