Data/hora: 05/06/2024 14:02:48

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression
Autores: | Lima,Juliana Oliveira; Pereira,Jorge Fernando; Araújo,Elza Fernandes de; Queiroz,Marisa Vieira de.
Data: | 2017-07-01
Ano: | 2017
Palavras-chave: | 2-Deoxy-d-glucose; Ultraviolet mutagenesis; Pectinolytic complex; Spontaneous mutant.
Resumo: | Abstract Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants were obtained. Some mutants produced sectors (segments morphologically different) that were also studied. The mutants were analyzed for pectinases production on pectinase-agar plates and five mutants and two sectors showing larger clearing zones than the wild type were selected for quantitative assay. Although PL production higher than the wild type has been found, phenotype instability was observed for most of the mutants and, after transfers to nonselective medium, the DG resistance was no longer present. Only mutants M03 and M04 were stable maintaining the DG-resistance phenotype. When growing for 120 h in liquid medium containing glucose with or without pectin, both mutants showed higher PL production. In the presence of glucose as sole carbon source, the mutant M03 produced 7.8-fold more PL than the wild type. Due its phenotypic stability and PL overproduction, the mutant M03 presents potential for industrial applications.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300602
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2016.12.009
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.48 n.3 2017
Fonte: | info:eu-repo/semantics/openAccess