Data/hora: 09/06/2024 03:45:41

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Further characterization of o-nitrobenzaldehyde degrading bacterium Pseudomonas sp: ONBA-17 and deduction on its metabolic pathway
Autores: | Yu,Fang-Bo; Li,Xiao-Dan; Ali,Shinawar Waseem; Shan,Sheng-Dao; Luo,Lin-Ping; Guan,Li-Bo.
Data: | 2014-12-01
Ano: | 2014
Palavras-chave: | Pseudomonas putida; Biodegradation; Metabolic pathway; Nitroaromatics.
Resumo: | A previously reported o-nitrobenzaldehyde (ONBA) degrading bacterium Pseudomonas sp. ONBA-17 was further identified and characterized. Based on results of DNA base composition and DNA-DNA hybridization, the strain was identified as P. putida. Its degradation effect enhanced with increase of inoculum amount and no lag phase was observed. Higher removal rate was achieved under shaking conditions. All tested ONBA with different initial concentrations could be completely degraded within 5 d. In addition, degradative enzyme(s) involved was confirmed as intra-cellular distributed and constitutively expressed. Effects of different compounds on relative activity of degradative enzyme(s) within cell-free extract were also evaluated. Finally, 2-nitrobenzoic acid and 2, 3-dihydroxybenzoic acid were detected as metabolites of ONBA degradation by P. putida ONBA-17, and relevant metabolic pathway was preliminary proposed. This study might help with future research in better understanding of nitroaromatics biodegradation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400021
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822014000400021
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.45 n.4 2014
Fonte: | info:eu-repo/semantics/openAccess