Data/hora: 13/06/2024 00:40:11

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation	
Autores: 	Sotero-Martins,Adriana; Bon,Elba Pinto da Silva; Carvajal,Elvira.	
Data: 	2003-12-01	
Ano: 	2003	
Palavras-chave: 	Asparaginase II; Nitrogen regulation; Saccharomyces cerevisiae; Genomic integration; Green fluorescent protein.	
Resumo: 	Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying asparaginase II secretion under nitrogen starvation in vivo.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822003000400017	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.34 n.4 2003	
Fonte: 	info:eu-repo/semantics/openAccess