Data/hora: 09/06/2024 11:46:50

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
Autores: | Sotero-Martins,Adriana; Bon,Elba Pinto da Silva; Carvajal,Elvira.
Data: | 2003-12-01
Ano: | 2003
Palavras-chave: | Asparaginase II; Nitrogen regulation; Saccharomyces cerevisiae; Genomic integration; Green fluorescent protein.
Resumo: | Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying asparaginase II secretion under nitrogen starvation in vivo.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822003000400017
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.34 n.4 2003
Fonte: | info:eu-repo/semantics/openAccess