Data/hora: 01/06/2024 15:44:13

Provedor de dados: 	Brazilian Journal of Microbiology	
País: 	Brazil	
Título: 	Genome-wide gene expression patterns in dikaryon of the basidiomycete fungus Pleurotus ostreatus	
Autores: 	Liu,Tianxiang; Li,Huiru; Ding,Yatong; Qi,Yuancheng; Gao,Yuqian; Song,Andong; Shen,Jinwen; Qiu,Liyou.	
Data: 	2017-06-01	
Ano: 	2017	
Palavras-chave: 	Differential gene expression; Monoallelic expression; Monokaryon; RNA editing; RNA-Seq.	
Resumo: 	Abstract Dikarya is a subkingdom of fungi that includes Ascomycota and Basidiomycota. The gene expression patterns of dikaryon are poorly understood. In this study, we bred a dikaryon DK13 × 3 by mating monokaryons MK13 and MK3, which were from the basidiospores of Pleurotus ostreatus TD300. Using RNA-Seq, we obtained the transcriptomes of the three strains. We found that the total transcript numbers in the transcriptomes of the three strains were all more than ten thousand, and the expression profile in DK13 × 3 was more similar to MK13 than MK3. However, the genes involved in macromolecule utilization, cellular material synthesis, stress-resistance and signal transduction were much more up-regulated in the dikaryon than its constituent monokaryons. All possible modes of differential gene expression, when compared to constituent monokaryons, including the presence/absence variation, and additivity/nonadditivity gene expression in the dikaryon may contribute to heterosis. By sequencing the urease gene poure sequences and mRNA sequences, we identified the monoallelic expression of the poure gene in the dikaryon, and its transcript was from the parental monokaryon MK13. Furthermore, we discovered RNA editing in the poure gene mRNA of the three strains. These results suggest that the gene expression patterns in dikaryons should be similar to that of diploids during vegetative growth.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200380	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2016.12.005	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.48 n.2 2017	
Fonte: 	info:eu-repo/semantics/openAccess