Data/hora: 13/06/2024 04:42:02

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Validated microbiological and HPLC methods for the determination of moxifloxacin in pharmaceutical preparations and human plasma	
Autores: 	Abdelaziz,Ahmed A.; Elbanna,Tarek E.; Gamaleldeen,Noha M.	
Data: 	2012-12-01	
Ano: 	2012	
Palavras-chave: 	Moxifloxacin; Microbiological assay; HPLC assay; Pharmaceutical analysis; Pharmacokinetics.	
Resumo: 	The article presents a comparison between microbiological and high performance liquid chromatographic (HPLC) assays for quantification of moxifloxacin in tablets, ophthalmic solutions and human plasma. The microbiological method employed a cylinder-plate agar diffusion assay using a strain of Esherichia coli ATCC 25922 as the test organism and phosphate buffer (pH8) as the diluent. The calibration curves were linear (R²&amp;gt; 0.98) over a concentration range of 0.125 to 16 µgml-1. The within day and between days precisions were &lt; 4.47% and &lt; 6.39% respectively. Recovery values were between 89.4 and 110.2%. The HPLC assay used Hypersil® BDS C18 reversed phase column (250×4.6 mm, 5µm) with a mobile phase comprising 20 mM ammonium dihydrogen orthophosphate (pH3) and acetonitrile (75:25) and flowing at 1.5 ml/min. The detection was at 295nm. The calibration curves were linear (R²&amp;gt; 0.999) over the range of 0.125 to 16 µg ml-1. The within day and between days precisions were &lt; 4.07% and &lt; 5.09% respectively. Recovery values were between 97.7 and 107.6%. Similar potencies were obtained after the analysis of moxifloxacin tablets and ophthalmic solutions by both methods. Also pharmacokinetic parameters were calculated after the analysis of plasma samples of six male healthhy volunteers by both validated methods.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400008	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822012000400008	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.43 n.4 2012	
Fonte: 	info:eu-repo/semantics/openAccess