Data/hora: 10/06/2024 09:19:24

Provedor de dados: | Brazilian Journal of Microbiology
País: | Brazil
Título: | Effects of phosphorus on polyphosphate accumulation by Cunninghamella elegans
Autores: | Lima,Marcos Antonio Barbosa de; Nascimento,Aline Elesbão do; Souza,Wanderley de; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de.
Data: | 2003-12-01
Ano: | 2003
Palavras-chave: | Polyphosphate; Accumulation; Ultrastructural cytochemistry; Cunninghamella elegans; Ortophosfate.
Resumo: | The content of inorganic polyphosphate and the polymeric degree of these compounds were evaluated during the growth of Cunninghamella elegans in medium containing varying orthophosphate (Pi) concentrations. For this purpose, a combination of chemical methods for polyphosphate extraction and ultrastructural cytochemistry were used. The orthophosphate and glucose consumption was also determined during the fungal cultivation. At Pi concentrations of 0.5, 2.5 and 0.0 g/L, the maximum amounts of biomass were 3.18, 3.29 and 0.24 g/L, respectively. During growth the cells accumulated Pi from the medium. At three days of growth the biomass consumed up to 100 and 95% of Pi from the media at initial concentrations of 0.5 and 2.5 g/L, respectively. Polyphosphate was observed at different Pi concentrations in medium and at different stages of growth. Polyphosphate was assayed by the content of labile phosphorus in water, acid-insoluble and alkali-soluble fractions. The content of fractions changed according to phosphorus concentration in the media and growth phase. During growth on all three media used, the cytochecmical behavior of polyphosphate changed considerably. The results obtained in this study reveal a potential of Cunninghamella elegans in the polyphosphate accumulation, and suggest a future application in the biotechnological processes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400016
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822003000400016
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.34 n.4 2003
Fonte: | info:eu-repo/semantics/openAccess