Data/hora: 09/06/2024 14:52:07

Provedor de dados: | 58
País: | Brazil
Título: | Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus
Autores: | Machado,Kátia Maria Gomes; Matheus,Dácio Roberto.
Data: | 2006-12-01
Ano: | 2006
Palavras-chave: | Peroxidases; Laccase; Solid-state fermentation; "shimeji" culture residue.
Resumo: | Pleurotus ostreatus ("shimeji") is produced in Brazil on a commercial scale using various lignocellulosic residues. Efforts have been made to reuse the culture residue to obtain products of greater aggregate value such as enzymes or in processes of bioremediation. We evaluated the Remazol brilliant blue R (RBBR) degradation potential of extracts from solid substrate colonized by P. ostreatus and extracts from residue of the "shimeji" mushroom yield. Colonized substrates and residue were provided by Toyobo do Brasil Ltda. Extraction was performed with sodium acetate buffer (50 mM, pH 4.6). RBBR decolorization was monitored at 592 nm and peroxidase and laccase activities were measured by monitoring the oxidation of ABTS. Horseradish peroxidase was used as reference. The time of growth of P. ostreatus influenced RBBR degradation and peroxidase and laccase activities. Concentration of 1 mM H2O2 and pH 4.0 were the best for RBBR decolorization. Complete RBBR decolorization was obtained with the addition of only one aliquot of 50 µL of 1 mM H2O2. The stability of the extracts was higher when they were kept under refrigeration than when stored frozen. The potential application of the ligninolytic complex derived from P. ostreatus and mushroom residue for xenobiotic degradation was demonstrated.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400013
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822006000400013
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.37 n.4 2006
Fonte: | info:eu-repo/semantics/openAccess