Data/hora: 13/06/2024 04:29:02

Provedor de dados: | 58
País: | Brazil
Título: | Yeast biomass production: a new approach in glucose-limited feeding strategy
Autores: | Vieira,Érika Durão; Andrietta,Maria da Graça Stupiello; Andrietta,Silvio Roberto.
Data: | 2013-01-01
Ano: | 2013
Palavras-chave: | Yeast; Biomass; Fed-batch; Process simulation; Saccharomyces cerevisiae.
Resumo: | The aim of this work was to implement experimentally a simple glucose-limited feeding strategy for yeast biomass production in a bubble column reactor based on a spreadsheet simulator suitable for industrial application. In biomass production process using Saccharomyces cerevisiae strains, one of the constraints is the strong tendency of these species to metabolize sugars anaerobically due to catabolite repression, leading to low values of biomass yield on substrate. The usual strategy to control this metabolic tendency is the use of a fed-batch process in which where the sugar source is fed incrementally and total sugar concentration in broth is maintained below a determined value. The simulator presented in this work was developed to control molasses feeding on the basis of a simple theoretical model in which has taken into account the nutritional growth needs of yeast cell and two input data: the theoretical specific growth rate and initial cell biomass. In experimental assay, a commercial baker's yeast strain and molasses as sugar source were used. Experimental results showed an overall biomass yield on substrate of 0.33, a biomass increase of 6.4 fold and a specific growth rate of 0.165 h-1 in contrast to the predicted value of 0.180 h-1 in the second stage simulation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200035
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822013000200035
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.44 n.2 2013
Fonte: | info:eu-repo/semantics/openAccess