Data/hora: 08/06/2024 11:00:20

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Short interfering RNAs targeting a vampire-bat related rabies virus phosphoprotein mRNA	
Autores: 	Ono,Ekaterina Alexandrovna Durymanova; Taniwaki,Sueli Akemi; Brandão,Paulo.	
Data: 	2017-07-01	
Ano: 	2017	
Palavras-chave: 	Rabies; Treatment; RNA interference.	
Resumo: 	Abstract The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3):879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus vampire bat) were used to inoculate BHK-21 cells and mice, and the transfection with each of the siRNAs was made with Lipofectamine-2000™. In vitro results showed that siRNA 360 was able to inhibit the replication of strain RABV-4005 with a 1 log decrease in virus titter and 5.16-fold reduction in P mRNA, 24 h post-inoculation when compared to non-treated cells. In vivo, siRNA 360 was able to induce partial protection, but with no significant difference when compared to non-treated mice. These results indicate that, despite the need for improvement for in vivo applications, P mRNA might be a target for an RNAi-based treatment for rabies.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300566	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2016.11.007	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.48 n.3 2017	
Fonte: 	info:eu-repo/semantics/openAccess