Data/hora: 13/06/2024 00:59:06

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis	
Autores: 	Chies,Jocelei Maria; Dias,Ana Christina de Oliveira; Maia,Hélio Mauro Moreira; Braga,Luis Paulo da Silva; Astolfi-Filho,Spartaco.	
Data: 	2006-12-01	
Ano: 	2006	
Palavras-chave: 	Type II restriction endonuclease; BliAI; ClaI; Isoschizomers; Bacillus licheniformis.	
Resumo: 	The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5'- AT&lt;FONT FACE=Symbol&gt;¯&lt;/FONT&gt;CGAT - 3', was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400027	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822006000400027	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.37 n.4 2006	
Fonte: 	info:eu-repo/semantics/openAccess